Coding

Part:BBa_K539713

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-20)

AlsS (alpha-acetolactate synthase protein)

This is the coding region of alsS from Bacillus subtilis. It encodes alpha-acetolactate synthase protein which is part of isobutanol biosynthesis pathway. Following is the isobutanol biosynthesis pathway.
Pathway%28small%29.jpg
Our team has mutated the function of RE site within the original alsS .
Alss-2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 308
    Illegal BsaI.rc site found at 902


[edit]
Categories
Parameters
None